In vitro evaluation of Leonotis nepetifolia L. and Ocimum gratissimum L. plant extracts against Colletotrichum lindemuthianum
Abstract
149 words
The potential of crude plant extracts as antimicrobial agents has been demonstrated both in
animal and plant health. Extracts with potential for control of plant pathogens such as
Colletotrichum lindemuthianum (causal agent for bean anthrocnose) which in many cases
require chemical control are a necessary component in plant protection regimes. The objective
of this study was to evaluate the efficacy of Leonotis nepetifolia L. (LN) and Ocimum
gratissimum L (OG) extracts to control Colletotrichum lindemuthianum in vitro. Composite
plant parts were shade-dried, extracted using methanol and used for the exploratory laboratory
bioassays. Preliminary results show that methanol extracts of LN and OG at concentration of
20.0 w/v produced inhibition results that were comparable to the synthetic fungicide, Rodazim 50SC at 20 ml/20l, with minimum inhibitory concentration (MIC) of 1.25mg/ml. These results will be used for levels bioassay studies of LN and OG extracts as potential antifungal agents against anthracnose.
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