Working Document Series Open Access 2010 RUF-34616

Importance and approaches of obtaining experimental uniformity

P. Gibson

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Abstract

119 words

The need for a high degree of experimental uniformity is clearly shown by the fact in an experiment that results in a CV (coefficient of variation) of 20%, 8 replications would be required to detect the size of difference between treatments that could be detected by 2 replications if the CV were 10%. Realistically, only if the percentage difference between two treatments is twice the magnitude of the CV, will the difference be reliably detected at P<0.05. Detection of differences can be enhanced by choosing the treatments carefully, using the most effective experimental design, conducting the experiment and gathering data with approaches that purposely minimize non-treatment differences between units, and analyzing the results with the best statistical approaches available.

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