Maize streak virus (MSV) diversity in Uganda and the assessment of gene silencing as a tool for development of resistance to MSV
Abstract
139 words
Through an extensive survey conducted in all maize-growing
regions in Uganda as well as the application of gene silencing
to develop a novel disease control option for maize streak
disease, the diversity of Maize streak virus (MSV) in different
regions in the country as well as a new disease resistance source
were established. Sequence data indicated that all Ugandan
MSV isolates belong to the maize-infecting MSV-A1 subtype
that is predominant in many sampled areas in East Africa and
that both inter- and intra-subtype recombination is common in
Ugandan MSVs. Using a transient assay, it was established
that MSV replication was abolished or greatly inhibited by a
hairpin of a 678-bp portion of an intronless MSV REP gene.
The rep hairpin construct also significantly reduced replication
of three southern African isolates as well as Ugandan isolates
identified in this study.
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