Working Document Series Open Access 2010 RUF-35236

Determination of genetic structure of germplasm collections: Are traditional hierarchical clustering methods appropriate for genetic marker data?

J. JansenT.L. OdongF.A. van EeuwijkJ. van HeerwaardenT.J.L. van Hintum

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Abstract

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We studied the performance of traditional hierarchical clustering techniques using molecular marker data. In this study, we showed that the cophenetic correlation coefficient is directly related to subgroup differentiation and can thus be used as an indicator of the presence of genetically distinct subgroups in germplasm collections. Whereas UPGMA performed well in preserving distances between accessions, Ward excelled in recovering groups. Our results also showed a close similarity between clusters obtained by Ward and by model-based cluster method (STRUCTURE). Traditional cluster analysis can provide an easy and effective way of determining structure in germplasm collections using molecular marker data.

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