Enhancing Callus induction and embrogenic cell suspension development in East African highland banana
Abstract
110 words
Improvement of banana using conventional breeding methods
is very difficult due to its biology. Genetic transformation is a
novel alternative approach for crop improvement.
Transformation of EA-AAA bananas has, however, been
limited by difficulties in callus induction and embryogenic cell
suspension (ECS) development. The existing protocols are not
readily responsive. In this study N-phenyl-N’-1,2,3-thidiazol-5ylurea
(TDZ) and N-(2-chloro-4-pyridyl)-N-phenylurea (4CPPU)
that are highly active in regulating morphogenesis in
the tissue culture of many plant species have been applied to
develop embryogenic callus and ECS culture systems with
reliable regeneration efficiency to enhance genetic improvement
and propagation of EA-AAA bananas. Preliminary observations
indicate that most growth regulator combinations have potential
for Callus induction.
Subjects
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