High Frequency Adventitious Root and Shoot Regeneration in Recalcitrant Sweet Potato Cultivars
Abstract
134 words
resistance to weevils through conventional hybridisation has
been limited by the lack of resistance sources in the sweetpotato
germplasm. The aim of this study was to investigate induction
of adventitious shoots which would facilitate genetic
transformation of I. batatas with weevil resistance genes.
Media with different concentrations of thidiazuron alone or in
combination with an auxin were used to investigate induction
of adventitious shoots from whole leaves and stem internode
segments of two I. batatas cultivars, Kyebandula and Bwanjule.
Shoot regeneration was successfully achieved from the two
explants of both cultivars. Statistical analysis showed that type
of explant effect was significant (P < 0.05) for regenerating
shoots. The results of this study are important for I. batatas
breeding This is particularly important for African cultivars which
have hitherto been difficult to regenerate in vitro.
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