Working Document Series Open Access 2012 RUF-32525

Mass propagation of selected papaya planting materials through shoot tip culture in Kenya

A.W. KihuraniE.G. MamatiN.N. MumoF.K.R. Wanzala

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Abstract

108 words

The objective of this study was to optimise media conditions in order to develop an efficient plantlet regeneration system of papaya planting materials. Twelve combinations of different levels of BAP (0.1, 0.3, 0.5 & 0.7mg/L) and NAA (0.05, 0.1 & 0.2mg/L) were used for shoot regeneration. The highest rate of shoot multiplication was recorded in 0.5mg/L BAP combined with 0.1 mg/L NAA. On the other hand, 0.1mg/L BAP combined with 0.05mg/L NAA produced the longest shoots in the three local papaya varieties. 2.5mg/l IBA induced about 85% of the shoots to root. Thus, an efficient plantlet regeneration system for Kenyan papaya through shoot tip culture was successfully developed.

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