Working Document Series Open Access 2010 RUF-35039

Characterisation of wild and cultured Oreochromis shiranus in Malawi

I. Changadeya & KosgeyD. KassamC.S. LewuantaiA. Maluwa

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Abstract

101 words

Oreochromis shiranus is a Malawi indigenous tilapia fish species. The aim of the study is to characterise the species phenotypically and genotypically within and between wild strains, unimproved and improved cultured strains. Phenotypic characterisation studies will be carried out using whole fish specimens while genotypic variation analysis will be done through DNA extraction, PCR and electrophoresis processes using fin-clips/ body tissue preserved in absolute alcohol with the help of gene markers. It is hoped that the results will be incorporated in a breeding programme to improve stunted and slow growth presently reported in many culture practices of O. shiranus in Malawi.

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