Enhancing soybean rust resistance through gene pyramiding
Abstract
167 words
The threat posed by soybean rust (Phakopsora pachyrhizi)
on soybean production is worsened by resistance breakdown
associated with single gene resistance present in most cultivars.
Few studies have, however, been undertaken to use mapped
simple sequence markers for gene pyramiding to enhance rust
resistance. This study validated use of identified simple
sequence repeat markers for gene pyramiding and determined
the most effective pairwise gene combination for three
independent soybean rust resistance genes, Rpp2, Rpp3 and
Rpp4. In the F2 generation, soybean plants (homozygous
dominant or heterozygous at both loci) with two gene
combinations had relatively lower disease severity and
sporulation than the parents suggesting complementary epistatic
gene action for resistance. Similarly, homozygous F3 families
showed lower severity, lesion density and sporulation. Gene
Rpp3 contributed positively to resistance with various genetic
backgrounds for most parameters measured compared to Rpp2
and Rpp4 resistance genes. Overall, the results suggest that
marker gene pyramiding is feasible and can substantially
increase resistance to soybean rust through reduced severity
and reduced sporulating lesions.
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