Working Document Series Open Access 2014 RUF-34149

Identifying conditions for phytase enzyme adaption in African catfish feeds

H. KasigwaC. KemigaboC. MasembeD. Sikawa

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Abstract

238 words

This research was conducted to establish and streamline conditions and optimum amount of phytase enzyme required for its adoption for improved digestibility of plant based catfish feeds. This started with analysis of pH along African catfish (Claria gariepinus) gastrointestinal tract (GIT), feed pH, phytic acid content and proximate nutrient composition. Results indicated that phytase enzyme from the fungus Aspergillus niger could be appropriate for adoption. A feeding experiment was conducted for one month and consisted factorial combination of four phytase enzyme activity levels (0, 750, 1000 and 1250 phytase units (FTUs) in feeds at four crude protein percentages (30%, 35%, 40% and 45%) fed to 10g catfish fingerlings reared in 5L indoor glass tanks .Faecal samples were collected from each tank by siphoning one hour after every feeding, frozen and later pooled .They were finally dried and apparent digestibility coefficients (% ADC) calculated by the indirect method for protein, dry matter and energy after proximate composition analysis. Data was analysed by multiple- regression to find the right model of factors influencing digestibility followed by ANOVA to identify differences among treatments and family wise differences determined by contrasts using R console statistical software version 3.0.2. The highest digestibility was observed at the activity unit of 1000 in all protein levels followed by 750FTUs, 1250FTUs and 0 FTUs (control). It was concluded that 1000FTUs is a suitable activity unit for increased digestibility of plant based fish feeds Uganda’s African catfish.

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