Working Document Series Open Access 2010 RUF-34558

Characterization of a diverse set of maize germplasm for resistance to infection by Aspergillus flavus and accumulation of aflatoxin

G. AseaD.B. KwemoiP. Okori

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Abstract

140 words

Sources of resistance to A. flavus have been identified elsewhere but no such materials are currently available for use by the maize improvement program in Uganda. The goal of this study was to initiate breeding for resistance to the fungus by identifying maize inbred lines, testers and hybrids that show high resistance to Aspergillus flavus and field accumulation of aflatoxin through: (1) screening inbred lines, testers and hybrids for resistance, (2) determining general and specific combining ability associated with resistance and determining the relationship between traits associated with resistance. Results indicated a highly significant variation (P<0.001) in resistance among inbred lines and testers. Testcrosses also showed significant variability (P<0.05). Inbred lines showed a significant GCA for kernel infection rate while testers showed significant GCA for severity of infection (P<0.05). SCA for both kernel infection rate and severity was non significant.

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