Evaluation of cassava genotype response to hot water therapy for efficient cassava virus elimination
Abstract
112 words
Cassava Brown Streak (CBSV) and Cassava Mosaic Viruses (CMV) are serious constraints to cassava production. In this work, the rate of shoot regeneration and disease incidence on shoots following hot water therapy as a technique for virus elimination was evaluated. RT-PCR was used to detect the presence of CBSV and CMV using the CTAB extraction protocol. Stem cutting of cultivars whose leaves were confirmed positive with RT PCR were subjected to hot water treatment at different temperature ranges and time intervals. Shoot regeneration and symptom appearance after thermotherapy treatment was studied and analyzed. All the shoots tested positive for both CMV and CBSV, with 40% exhibiting co-infected with both CBSV and CBSV-Ug.
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