Working Document Series Open Access 2012 RUF-31909

Diversity and Symbiotic Effectiveness of Acid Tolerant Groundnut Rhizobia inWestern Kenya Soils

J.K. OgegaJ.R. OkaleboD.A. OnyangoB.A. Were

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Abstract

106 words

This study was carried out to find suitable strains of rhizobia for use in ground nut production under acid-aluminium soils. Thirty-six rhizobia isolates tolerant to high soil aluminium were identified.. The isolates were gram negative rod cells that reduced nitrate to nitrite, urea to ammonia and utilized citrate. Three isolates A6w, W1w and V2w were selected as the most promising for efficient Biological Nitrogen Fixation (BNF) in groundnut. Upon testing them for symbiotic effectiveness on a groundnut monocrop, V2w and A6w had the best performance compared to Biofix, a commercial strain. These two have been converted to a bio-fertilizer form that is under trial by farmers.

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