Working Document Series Open Access 2012 RUF-32466

Establishment of a Rapid in Vitro System for Regeneration of Papaya Plantlets in a Liquid Media

E.K. GatambiaA.W. KihuraniM.M. WaiganjoF.K.R. Wanzala

Download Read online

Abstract

107 words

This study was an attempt to determine rapid micro- propagation methods for regeneration of papaya plantlets in vitro using liquid media. Different concentrations and combinations of growth regulators in liquid MS namely BAP (0-2 mgl-1) and NAA (0-1 mgl-1), CPPU (0-2 mgl-1), and IBA (0-3 mgl-1) were used in the experiments. Results showed that 0.2 mgl-1 BAP, 0.5mgl CPPU were best in shoot multiplication, while 3.0 mg l-1 of IBA in root initiation. Shoot proliferation, root induction reported in this study is an indicator that liquid medium could be a rapid method for papaya regeneration. Further experiments geared towards optimisation of shoot elongation and rooting are ongoing.

Subjects

1 category