Aphid transmission and alternate hosts of Passiflora chlorotic mottle virus in Uganda
Abstract
105 words
This research established aphid vectors and alternate hosts of Passiflora chlorotic mottle
virus in Uganda. Aphids and host plants were collected from farmers’ fields in major passion
fruit growing districts in Uganda. Transmission tests were carried out in passion fruit and
Nicotiana benthamiana using the aphids. Double antibody sandwich enzyme-linked
immunosorbent assays (DAS-ELISA) and reverse transcription polymerase chain reactions
were carried out to confirm virus presence. Results reveal cowpea aphid (Aphis craccivora),
bean aphid (Aphis fabae) and green peach aphid (Myzus persicae) as efficient vectors of
the virus. Common bean, cowpea, black jack, spiny amaranth and spinach were also good
alternate hosts for the virus.
Subjects
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