Working Document Series Open Access 2010 RUF-34552

Detection and screening of yellow and sweet calabash passion fruit for resistance to woodiness disease in Uganda

G. DdamuliraR. EdemaS. MusaanaM.K.N. Ochwo-SsemakulaP. Sseruwagi

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Abstract

96 words

In Uganda passion fruit woodiness disease (PWD) damages passion fruit, causing yield losses of 60-100%. This study aimed at detecting and screening yellow and sweet calabash passion fruit for resistance to PWD. Four viruses, namely, Cowpea aphid borne mosaic virus, Cucumber mosaic virus, Passiflora latent virus, and Tomato ringspot virus were detected using ELISA. Only Cowpea Aphid borne mosaic virus showed a weak reactivity with antiserum, indicating its presence in the tested samples in low concentrations. The detected virus was used to mechanically inoculate yellow and sweet calabash to observe symptom development. This is still on-going.

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