Molecular Characterisation of Multidrug Resistant Tuberculosis Human Isolates in Kampala
Abstract
173 words
Strains of Mycobacteria tuberculosis complex that are resistant to the two first line drugs
rifampicin and isoniazid are increasing in Kampala. Archived human multidrug resistant
tuberculosis isolates collected from Kampala between 1997 and 2007 were analysed. We
used regions of deletion to determine the species involved. Spoligotyping was employed
to identify strain types in relation to the international SpolDB4 database. Epidemiological
linkages between isolates were also ascertained by comparing the numerical 15-loci
MIRU-VNTR outputs to the MIRU-VNTRPlus database to elucidate clustered strains.
The predominant species were M. tuberculosis of the “modern” type (98.7%), “ancestral”
M. tuberculosis (1.03%) and M. bovis “classical” (1.03%). A strain diversity of 46.9%
was revealed by SpolDB4. Predominant spoligotype strain lineages were T2-Uganda
(20.4%) and CAS1-Delhi (12.2%). Spoligotype clusters were split by the 15-loci MIRUVNTR
typing, forming two MIRU-VNTR clusters each having two strains. Predominant
MIRU-VNTR strain lineages were Uganda I (29.6%) and CAS1- Dehli (11.2%). Majority
(98%) of the strains were unclustered.
These data suggest that MDR-TB strains circulating in Kampala are endemic, genetically
diverse and epidemiologically independent.
Subjects
1 categoryRelated papers
More in Theses & Dissertations →- The role of agrifood systems in addressing obesity and overweight in Africa
- Black soldier fly larvae meal as a cheap alternative to expensive conventional protein sources in poultry feeds
- Sugar Content and Physical Characterization of Four Selected African Nightshade (Solanum nigrum) Edible Berries
- Effect of the consumption of fermented sorghum porridge fortified with moringa leaf powder and baobab fruit pulp on the nutritional status of children aged 6 to 59 months in northern Benin