Analysis of drought tolerance in selected upland rice genotypes in Uganda
Abstract
186 words
Breeding for drought tolerance requires a clear understanding
of the mode of gene action for this trait. To understand the
action of drought tolerance genes in upland rice varieties in
Uganda, a study was carried out to establish the general and
specific combining ability of the new rice lines acquired in the
breeding programme as well as validate QTLs from previous
studies in our own population and environment. F2 populations
from five susceptible and five resistant parents were generated.
Drought was simulated in the screenhouse by restricted watering
at 50% tillering. Vegetative stage data were taken on leaf rolling,
leaf drying, tiller number, plant height flag leaf length and width.
Reproductive stage data were taken on days to flowering, grain
filling and yield per plant. Molecular analysis to identify known
quantitative trait loci (QTL) for drought tolerance in F2s was
performed using 27 SSR markers. Seven markers have been
found polymorphic on four parents. Results on general and
specific combining ability of the tolerance genes are expected
from comparative studies on parents, and F2 populations.
Candidate QTLs are expected from a screening of one F2
population.
Subjects
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