Working Document Series Open Access 2012 RUF-31504

Marker Assisted Introgression of Phytophthora capsici Resistance Genes into Locally Adapted Pepper Variety cv. Scotch Bonnet

E. AworiS.T. NkaluboG. Tusiime

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Abstract

186 words

Phytophthora capsici, a soil born fungus, is one of the most important constraint to hot pepper production in Uganda, causing root rot and wilt disease. Current management approaches that are mainly cultural and chemical do not provide adequate protection against the disease. Consequently, a lot of the crop is lost. This study is broadly aiming at developing resistance against the disease. Specific objectives of the study are: (i) to test the effectiveness of molecular markers in aiding the introgression of the Phyto 5.2 QTL into susceptible varieties, and (ii) to determine the contribution of the Phyto 5.2 QTL towards resistance to P. capsici in pepper. A resistant line was crossed with a susceptible variety. F1s were screened using a SCAR and SSR markers reported to be linked to Phyto 5.2, a major QTL for resistance to the disease. A total of seven SSR markers were tested in addition to the SCAR. The SCAR marker was unfortunately not polymorphic, while only one SSR marker is promising. F2s and backcross 1 populations will be phenotypically evaluated to determine any relationship between observed resistance and presence of SSR marker.

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